Shy nature of mithun bull and a less pronounced estrous sign of mithun female make semen collection and artificial insemination (AI) in mithun a tiresome task. Nevertheless, AI is an important step for the implementation of any genetic improvement programme. A team of Scientists of Animal Physiology and Reproduction section developed a protocol for the freezing of mithun semen using controlled bio-freezer. In order to address the problem of detection of estrus in mithun, successfully validated the ‘Ovsynch’ protocol for timed AI. Subsequently, collection of semen using artificial vagina was also satandarized.
During this year, in order to implement 100% AI in the Mithun Farm, estrus synchronization and timed AI was introduced. Estrus synchronization of 28 mithun cows was carried out. Out of these animals 24 (85.71%) exhibited estrus behavior. 19 animals were inseminated with frozen thawed Mithun and 14 (73.68%) were confirmed as pregnant on the basis of nonreturn rate and per-rectal examination. Till March 2017, 11 AI inseminated cows were calved producing 09 female and 2 male calves. The efforts are also being made to implement AI in the farmers herd.