Artificial insemination (AI) technique in Mithun has been standardized and implemented successfully both in farm and field condition at NRC Mithun, Nagaland First Mithun calf under field condition was born on 6 May 2010 using a standardized AI technique at Mithun rearing area of Khonoma village in Kohima district of Nagaland. For AI, we require to have a standardized method of collection of semen and its preservation. The institute has been successfully standardized the collection procedure of semen with an innovative way of spraying urine of estrus females on the dummy cow for attracting the bulls. Moreover, the method of preservation of semen has also been standardized. Two Mithun cows were synchronised into oestrus by using the protocol developed at the Institute. Cryopreserved semen was used for insemination. The cows have conceived following insemination and one of them gave birth to a healthy male calf. Mr Selie Khate, the proud owner of cows is very happy and now approached us along with the other farmers to continue the programme in their village.